5D0E
Crystal Structure of an adenylyl cyclase Ma1120-Cat in complex with GTP and calcium from Mycobacterium avium
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-02-12 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.978560 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 52.590, 55.710, 55.270 |
Unit cell angles | 90.00, 109.74, 90.00 |
Refinement procedure
Resolution | 27.095 - 1.480 |
R-factor | 0.1907 |
Rwork | 0.189 |
R-free | 0.22540 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4wp9 |
RMSD bond length | 0.013 |
RMSD bond angle | 0.803 |
Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 27.100 | 1.560 |
High resolution limit [Å] | 1.480 | 1.480 |
Rmerge | 0.068 | 0.930 |
Number of reflections | 50213 | |
<I/σ(I)> | 13.2 | |
Completeness [%] | 99.8 | 99.1 |
Redundancy | 7.4 | 7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 7.5 | 291 | 0.1M HEPES, 30% (w/v) PEG 1000 |