5D0E
Crystal Structure of an adenylyl cyclase Ma1120-Cat in complex with GTP and calcium from Mycobacterium avium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-02-12 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.978560 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 52.590, 55.710, 55.270 |
| Unit cell angles | 90.00, 109.74, 90.00 |
Refinement procedure
| Resolution | 27.095 - 1.480 |
| R-factor | 0.1907 |
| Rwork | 0.189 |
| R-free | 0.22540 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4wp9 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 0.803 |
| Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.100 | 1.560 |
| High resolution limit [Å] | 1.480 | 1.480 |
| Rmerge | 0.068 | 0.930 |
| Number of reflections | 50213 | |
| <I/σ(I)> | 13.2 | |
| Completeness [%] | 99.8 | 99.1 |
| Redundancy | 7.4 | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 7.5 | 291 | 0.1M HEPES, 30% (w/v) PEG 1000 |
