5D0B

Crystal structure of epoxyqueuosine reductase with a tRNA-TYR epoxyqueuosine-modified tRNA stem loop

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-06-25
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9795
Spacegroup name	P 1 21 1
Unit cell lengths	45.644, 110.754, 99.223
Unit cell angles	90.00, 95.16, 90.00

Refinement procedure

Resolution	32.049 - 2.645
R-factor	0.1932
Rwork	0.192
R-free	0.22140
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5d08
RMSD bond length	0.004
RMSD bond angle	0.728
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	100.000	100.000	2.740
High resolution limit [Å]	2.645	5.710	2.650
Rmerge	0.154	0.102	0.337
Rmeas	0.169	0.112	0.392
Rpim	0.067	0.045	0.194
Total number of observations	138854
Number of reflections	26382
<I/σ(I)>	5.3
Completeness [%]	92.8	98.7	68.3
Redundancy	5.3	5.6	3.3
CC(1/2)		0.989	0.873

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5	294	(0.1 M acetate (pH 4.5), 0.4 M (NH4)H2PO4, 12% (w/v) PEG 3350