5D0A
Crystal structure of epoxyqueuosine reductase with cleaved RNA stem loop
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-04-17 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9792 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 90.587, 111.118, 96.008 |
Unit cell angles | 90.00, 104.90, 90.00 |
Refinement procedure
Resolution | 46.390 - 2.100 |
R-factor | 0.186 |
Rwork | 0.184 |
R-free | 0.22500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5d08 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.088 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.180 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.394 | |
Number of reflections | 104237 | |
<I/σ(I)> | 5.8 | |
Completeness [%] | 97.4 | 89.3 |
Redundancy | 3.4 | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 294 | (0.1 M acetate, 0.4 M (NH4)H2PO4, 12% (w/v) PEG 3350 |