5CZV
Crystal structure of Notch3 NRR in complex with 20350 Fab
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 17-ID |
Synchrotron site | APS |
Beamline | 17-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-11-28 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.000 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 91.917, 104.349, 92.849 |
Unit cell angles | 90.00, 113.17, 90.00 |
Refinement procedure
Resolution | 29.620 - 3.190 |
R-factor | 0.2062 |
Rwork | 0.204 |
R-free | 0.25650 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3eto |
RMSD bond length | 0.010 |
RMSD bond angle | 1.230 |
Data scaling software | SCALEPACK |
Refinement software | BUSTER-TNT |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 30.000 | 30.000 | 3.260 |
High resolution limit [Å] | 3.190 | 8.620 | 3.190 |
Rmerge | 0.126 | 0.078 | 0.272 |
Total number of observations | 29800 | ||
Number of reflections | 11614 | ||
<I/σ(I)> | 4.8 | ||
Completeness [%] | 86.4 | 93.2 | 79.1 |
Redundancy | 2.6 | 2.6 | 2.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 298 | 0.1M NaAc, pH 5.6, 17.5% (v/v) PEG3000 |