5CZG
Crystal Structure Analysis of hypothetical bromodomain Tb427.10.7420 from Trypanosoma brucei in complex with bromosporine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-07-10 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97921 |
| Spacegroup name | P 43 2 2 |
| Unit cell lengths | 36.766, 36.766, 162.210 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.766 - 1.451 |
| R-factor | 0.1649 |
| Rwork | 0.162 |
| R-free | 0.21380 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5ame |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.093 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.480 |
| High resolution limit [Å] | 1.450 | 3.940 | 1.450 |
| Rmerge | 0.050 | 0.025 | 0.295 |
| Rmeas | 0.054 | 0.028 | 0.324 |
| Rpim | 0.020 | 0.011 | 0.131 |
| Total number of observations | 152445 | ||
| Number of reflections | 20807 | ||
| <I/σ(I)> | 18.7 | ||
| Completeness [%] | 99.7 | 94.8 | 100 |
| Redundancy | 7.3 | 6.2 | 6 |
| CC(1/2) | 0.999 | 0.951 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 1.5M Sodium Citrate, 62.5 mM Tris-HC |
