5CZF
Crystal structure of the PaaA2-ParE2 antitoxin-toxin complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-05-24 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97911 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 91.560, 91.560, 185.600 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.777 - 2.671 |
| R-factor | 0.2035 |
| Rwork | 0.201 |
| R-free | 0.25210 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5cw7 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.611 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.780 | 2.767 |
| High resolution limit [Å] | 2.671 | 2.671 |
| Rmerge | 0.190 | 1.231 |
| Number of reflections | 13723 | |
| <I/σ(I)> | 17.62 | 2.5 |
| Completeness [%] | 99.9 | 99.33 |
| Redundancy | 20.4 | 17.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 293 | 100 mM sodium acetate pH 5.0, 1 M ammonium sulfate |
