5CZ8

Yeast 20S proteasome beta5-L(-49)S-K33A mutant in complex with Carfilzomib

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-07-19
Detector	PSI PILATUS 6M
Wavelength(s)	1.0
Spacegroup name	P 1 21 1
Unit cell lengths	136.440, 299.270, 144.460
Unit cell angles	90.00, 113.12, 90.00

Refinement procedure

Resolution	15.000 - 2.800
R-factor	0.19212
Rwork	0.191
R-free	0.21544
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ryp
RMSD bond length	0.004
RMSD bond angle	0.852
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	REFMAC
Refinement software	REFMAC (5.7.0032)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.900
High resolution limit [Å]	2.800	2.800
Rmerge	0.070	0.536
Number of reflections	251187
<I/σ(I)>	11.4	2.3
Completeness [%]	96.2	97.5
Redundancy	3.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.8	293	20 mM MgAC2, 13% MPD, 0.1 M MES