5CYX
Crystal Structure of Mouse Protocadherin-24 EC1-3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-07-10 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.97910 |
Spacegroup name | P 3 2 1 |
Unit cell lengths | 74.767, 74.767, 140.620 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 50.000 - 2.100 |
R-factor | 0.21148 |
Rwork | 0.210 |
R-free | 0.24692 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4aqe |
RMSD bond length | 0.014 |
RMSD bond angle | 1.632 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0124) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.140 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.337 | |
Number of reflections | 27017 | |
<I/σ(I)> | 27 | 4.4 |
Completeness [%] | 99.2 | 90.6 |
Redundancy | 8.6 | 5.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 3.0 M LiCl2 0.1 M HEPES pH 7.5 |