5CYN
JC Virus large T-antigen origin binding domain F258L mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-02-25 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.075 |
| Spacegroup name | I 41 |
| Unit cell lengths | 103.540, 103.540, 34.340 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.700 |
| R-factor | 0.19682 |
| Rwork | 0.195 |
| R-free | 0.22747 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4lif |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.190 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0107) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.120 | 0.660 |
| Number of reflections | 5163 | |
| <I/σ(I)> | 37.4 | 2.7 |
| Completeness [%] | 99.6 | 96.6 |
| Redundancy | 15.4 | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 0.1 M Tris pH 8.5, 25-35% Peg 6000 |
