5CY4

Crystal structure of an oligoribonuclease from Acinetobacter baumannii

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2015-07-07
Detector	RAYONIX MX225HE
Wavelength(s)	0.97872
Spacegroup name	P 21 21 21
Unit cell lengths	92.710, 102.880, 184.870
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.490 - 2.250
R-factor	0.1701
Rwork	0.169
R-free	0.20160
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3igi
RMSD bond length	0.004
RMSD bond angle	0.771
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.10pre_2090: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.000	2.310
High resolution limit [Å]	2.250	2.250
Rmerge		0.562
Number of reflections	82841
<I/σ(I)>	16.3	3.2
Completeness [%]	98.0	99.3
Redundancy	5	5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	JCSG+e11: 14% PEG8000, 160mM Calcium acetate, 0.08M sodium cacodylate pH6.5, 20% glycerol