5CXF
Crystal structure of the extracellular domain of glycoprotein B from Human Cytomegalovirus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-11-15 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97921 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 92.183, 133.930, 295.376 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 57.633 - 3.602 |
| R-factor | 0.2334 |
| Rwork | 0.232 |
| R-free | 0.26920 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3fvc |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.291 |
| Data reduction software | XDS |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 57.633 | 3.750 |
| High resolution limit [Å] | 3.600 | 3.600 |
| Rmerge | 0.167 | 0.961 |
| Number of reflections | 42580 | |
| <I/σ(I)> | 9 | 1.6 |
| Completeness [%] | 99.4 | 99.9 |
| Redundancy | 4.1 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | PEG 8000, tris, magnesium chloride, |
