5CWZ
Crystal structure of the kinase domain of human TRAF2 and NCK-interacting protein kinase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B2 |
Synchrotron site | SPring-8 |
Beamline | BL26B2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-04-21 |
Detector | RAYONIX MX-225 |
Wavelength(s) | 1.0 |
Spacegroup name | P 2 21 21 |
Unit cell lengths | 50.215, 123.896, 158.450 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.586 - 2.900 |
R-factor | 0.2123 |
Rwork | 0.209 |
R-free | 0.28140 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2x7f |
RMSD bond length | 0.004 |
RMSD bond angle | 0.791 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.590 | 3.080 |
High resolution limit [Å] | 2.900 | 2.900 |
Rmerge | 0.692 | |
Number of reflections | 22760 | |
<I/σ(I)> | 18.2 | 3.4 |
Completeness [%] | 100.0 | 100 |
Redundancy | 7.3 | 7.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.2 | 293 | 0.2M Ammonium sulfate, 0.1M Bis-tris (6.2), 35% PEG3350, 9% Ethylene glycol |