5CWE
Structure of CYP107L2 from Streptomyces avermitilis with lauric acid
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 5C (4A) |
| Synchrotron site | PAL/PLS |
| Beamline | 5C (4A) |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-07-22 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.97952 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 64.080, 118.371, 66.039 |
| Unit cell angles | 90.00, 105.51, 90.00 |
Refinement procedure
| Resolution | 47.430 - 2.390 |
| R-factor | 0.22961 |
| Rwork | 0.226 |
| R-free | 0.30085 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2cd8 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.727 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.440 |
| High resolution limit [Å] | 2.390 | 2.390 |
| Rmerge | 0.447 | |
| Number of reflections | 37158 | |
| <I/σ(I)> | 25.5 | 3.9 |
| Completeness [%] | 99.4 | 95.8 |
| Redundancy | 4 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 287 | Lithium sulfate monohydrate, Tris-HCL, PEG 3350 |
