5CVJ
Monolignol 4-O-methyltransferase 5 - coniferyl alcohol
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-03-20 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 66.070, 150.360, 67.930 |
| Unit cell angles | 90.00, 93.19, 90.00 |
Refinement procedure
| Resolution | 75.180 - 1.800 |
| R-factor | 0.15234 |
| Rwork | 0.151 |
| R-free | 0.18003 |
| RMSD bond length | 0.022 |
| RMSD bond angle | 2.176 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 75.180 | 1.770 |
| High resolution limit [Å] | 1.680 | 1.600 |
| Rmerge | 0.360 | |
| Number of reflections | 146779 | |
| <I/σ(I)> | 14.6 | 3.6 |
| Completeness [%] | 97.8 | 85.1 |
| Redundancy | 5.7 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 293 | Crystals of both MOMT5 were grown via a hanging-drop vapor diffusion method in 22% (w/v) PEG 4000, 0.3 M Mg(NO3)2 (pH 7.2), and 1 mM DTT with 1 mM SAH and 1 mM monolignols |
