5CV4
Crystal structure of Staphylococcal nuclease variant Delta+PHS V23D/V66H at cryogenic temperature
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | OTHER |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2015-01-28 |
| Detector | APEX II CCD |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 31.105, 60.691, 38.414 |
| Unit cell angles | 90.00, 93.58, 90.00 |
Refinement procedure
| Resolution | 38.340 - 1.800 |
| R-factor | 0.199 |
| Rwork | 0.197 |
| R-free | 0.23160 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3bdc |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.769 |
| Data reduction software | APEX |
| Data scaling software | APEX (2013/3 for Windows) |
| Phasing software | PHASER (2.5.7) |
| Refinement software | REFMAC (5.8.0124) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.830 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.024 | 0.156 |
| Total number of observations | 165622 | |
| Number of reflections | 13299 | |
| <I/σ(I)> | 28.43 | 5.84 |
| Completeness [%] | 100.0 | 99.8 |
| Redundancy | 11.46 | 6.05 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | 20% MPD, 25 mM potassium phosphate, calcium chloride, pdTp |
