5CTT
Crystal structure of human SART3/TIP110 NLS-mouse importin alpha complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 5C (4A) |
Synchrotron site | PAL/PLS |
Beamline | 5C (4A) |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-11-05 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9794 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 79.149, 89.992, 97.818 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.765 - 1.700 |
R-factor | 0.1918 |
Rwork | 0.191 |
R-free | 0.21890 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ial |
RMSD bond length | 0.007 |
RMSD bond angle | 1.094 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.7.3_928) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.760 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.078 | 0.453 |
Number of reflections | 77648 | |
<I/σ(I)> | 19.8 | 1.96 |
Completeness [%] | 99.0 | 97.4 |
Redundancy | 2.8 | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 100mM HEPES, pH 6.6, 1.6M sodium citrate, 10mM DTT |