5CRV

Crystal structure of the Bro domain of HD-PTP in a complex with the core region of STAM2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	93
Detector technology	CCD
Collection date	2014-11-20
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97949
Spacegroup name	P 1 21 1
Unit cell lengths	81.647, 37.218, 140.019
Unit cell angles	90.00, 103.41, 90.00

Refinement procedure

Resolution	29.089 - 2.001
R-factor	0.2137
Rwork	0.212
R-free	0.24080
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3rau
RMSD bond length	0.003
RMSD bond angle	0.701
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.8.2_1309)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.030
High resolution limit [Å]	2.000	2.000
Number of reflections	56289
<I/σ(I)>	30.4	4.6
Completeness [%]	97.3	95.5
Redundancy	4.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	0.1 M Bis-Tris, 30%(w/v) polyethylene glycol 3350, 4%(v/v) acetonitrile