5CQG
Structure of Tribolium telomerase in complex with the highly specific inhibitor BIBR1532
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-11-04 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 117.719, 84.902, 123.333 |
| Unit cell angles | 90.00, 116.20, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.300 |
| R-factor | 0.23696 |
| Rwork | 0.235 |
| R-free | 0.27326 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3du6 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.043 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.123 | 0.471 |
| Number of reflections | 97056 | |
| <I/σ(I)> | 4.3 | |
| Completeness [%] | 99.6 | 98.9 |
| Redundancy | 3 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 1.3M NaNO3 100mM Tris 8.5 |
