5CQ0
Crystal structure of murine polyomavirus RA strain VP1 in complex with the GD1a glycan
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-03-15 |
Detector | DECTRIS PILATUS 2M-F |
Wavelength(s) | 1.0 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 219.060, 219.060, 99.400 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 48.120 - 1.900 |
R-factor | 0.1553 |
Rwork | 0.155 |
R-free | 0.17840 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5cpu |
RMSD bond length | 0.008 |
RMSD bond angle | 1.192 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHENIX |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.950 |
High resolution limit [Å] | 1.900 | 1.900 |
Number of reflections | 212126 | |
<I/σ(I)> | 11.3 | 1.9 |
Completeness [%] | 99.0 | 99.1 |
Redundancy | 2.9 | 2.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 1.5 M ammonium sulfate, 6% (v/v) isopropanol |