5COV
Structure and mechanism of a eukaryal nick-sealing RNA ligase K170M+Mn
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-12-09 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.97920 |
Spacegroup name | P 32 |
Unit cell lengths | 55.479, 55.479, 98.551 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 27.740 - 2.200 |
R-factor | 0.19442 |
Rwork | 0.191 |
R-free | 0.26711 |
RMSD bond length | 0.012 |
RMSD bond angle | 1.610 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0123) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.290 | |
Number of reflections | 16304 | |
<I/σ(I)> | 22.1 | 2 |
Completeness [%] | 95.0 | 84.6 |
Redundancy | 2.3 | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 0.1 M HEPES, 30% PEG 6000, 1 mM Mn |