5COU
Structure and mechanism of a eukaryal nick-sealing RNA ligase K170M+ATP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-04-14 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.97921 |
Spacegroup name | P 32 |
Unit cell lengths | 54.969, 54.969, 100.308 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 24.100 - 1.900 |
R-factor | 0.17952 |
Rwork | 0.176 |
R-free | 0.24672 |
RMSD bond length | 0.019 |
RMSD bond angle | 2.081 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.950 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.437 | |
Number of reflections | 26788 | |
<I/σ(I)> | 27.1 | 3 |
Completeness [%] | 100.0 | 99.9 |
Redundancy | 7.1 | 7.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 0.1 M HEPES, 30% PEG 6000, 2 mM ATP |