5CNO

Crystal structure of the EGFR kinase domain mutant V924R

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2009-07-22
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9998
Spacegroup name	C 1 2 1
Unit cell lengths	155.400, 71.837, 76.399
Unit cell angles	90.00, 113.27, 90.00

Refinement procedure

Resolution	38.560 - 1.550
R-factor	0.1763
Rwork	0.175
R-free	0.20290
RMSD bond length	0.017
RMSD bond angle	1.677
Data reduction software	HKL-2000
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (dev_1839)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.560	1.605
High resolution limit [Å]	1.550	1.550
Number of reflections	110980
<I/σ(I)>	7.58	1.32
Completeness [%]	99.0	92
Redundancy	1.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	ammonium nitrate, PEG 3350