5CNJ

mGlur2 with glutamate analog

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 31-ID
Synchrotron site	APS
Beamline	31-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2011-01-18
Detector	RAYONIX MX225HE
Wavelength(s)	0.97931
Spacegroup name	P 21 21 2
Unit cell lengths	80.391, 155.087, 92.714
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.040 - 2.650
R-factor	0.1945
Rwork	0.193
R-free	0.24120
RMSD bond length	0.010
RMSD bond angle	1.120
Data reduction software	MOSFLM
Data scaling software	SCALA
Refinement software	BUSTER (2.11.5)

Data quality characteristics

	Overall
Low resolution limit [Å]	28.040
High resolution limit [Å]	2.650
Number of reflections	33677
<I/σ(I)>	7.6
Completeness [%]	97.9
Redundancy	6.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		295	100mM Hepes pH 7 + 20% PEG 3350 + 200mM Magnesium Chloride