5CN0

Artificial HIV fusion inhibitor AP2 fused to the C-terminus of gp41 NHR

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2013-04-08
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	H 3 2
Unit cell lengths	36.187, 36.187, 286.003
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	31.778 - 1.901
R-factor	0.1999
Rwork	0.196
R-free	0.23240
RMSD bond length	0.019
RMSD bond angle	1.561
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.8.2_1309)

Data quality characteristics

	Overall
Low resolution limit [Å]	31.780
High resolution limit [Å]	1.900
Number of reflections	64372
<I/σ(I)>	28.9
Completeness [%]	100.0
Redundancy	10.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	289	0.1M Tris-HCl pH 8.0, 34% (w/v) PEG 3350, 0.2M MgCl2