5CMS
Structural Insights into the Mechanism of Escherichia coli Ymdb
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-07-05 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9791 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 290.019, 290.019, 114.324 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 41.900 - 2.980 |
R-factor | 0.2231 |
Rwork | 0.222 |
R-free | 0.24970 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1spv |
RMSD bond length | 0.016 |
RMSD bond angle | 1.841 |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.050 |
High resolution limit [Å] | 2.980 | 3.000 |
Rmerge | 0.166 | 0.783 |
Number of reflections | 109753 | |
<I/σ(I)> | 14 | 2.8 |
Completeness [%] | 98.1 | 100 |
Redundancy | 10.6 | 10.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 285 | 2 M ammonium sulfate, 0.1 M BIS-TRIS |