5CMN
FLRT3 LRR domain in complex with LPHN3 Olfactomedin domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 295 |
Detector technology | CCD |
Collection date | 2014-12-12 |
Detector | ADSC QUANTUM 210r |
Wavelength(s) | 1.00883 |
Spacegroup name | P 43 |
Unit cell lengths | 102.268, 102.268, 419.230 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 73.196 - 3.605 |
R-factor | 0.1987 |
Rwork | 0.195 |
R-free | 0.26180 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4wxq |
RMSD bond length | 0.005 |
RMSD bond angle | 0.938 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 75.000 | 3.660 |
High resolution limit [Å] | 3.600 | 3.600 |
Rmerge | 0.229 | 0.568 |
Number of reflections | 45263 | |
<I/σ(I)> | 6.031 | 1.347 |
Completeness [%] | 92.0 | 60.6 |
Redundancy | 3.6 | 1.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 6 | 295 | 10% (w/v) PEG 3000, 100mM MES pH6.0, 200mM Lithium sulfate |