5CMM
Crystal structure of the GluK2EM LBD dimer assembly complex with 2S,4R-4-methylglutamate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-07-01 |
| Detector | RAYONIX MX300-HS |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 61 |
| Unit cell lengths | 52.308, 52.308, 170.923 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 27.287 - 1.271 |
| R-factor | 0.1441 |
| Rwork | 0.143 |
| R-free | 0.16320 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1s50 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.236 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.290 |
| High resolution limit [Å] | 1.270 | 1.270 |
| Rmerge | 0.043 | 0.335 |
| Number of reflections | 68757 | |
| <I/σ(I)> | 45.64 | 3.8 |
| Completeness [%] | 99.4 | 95.2 |
| Redundancy | 7 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 291 | Reservoir: 18% PEG 4K Protein buffer: 20 mM NaCl, 5 mM 2S,4R-4-methylglutamate, 1 mM EDTA, 2 mM TRIS pH 8 |
