5CMA
Anti-B7-H3 monoclonal antibody ch8H9 Fab fragment
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-08-02 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97950 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 64.576, 207.422, 85.491 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.001 - 2.500 |
| R-factor | 0.2106 |
| Rwork | 0.208 |
| R-free | 0.26060 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3d85 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.869 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP (11.0.02) |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 2.540 |
| High resolution limit [Å] | 2.500 | 6.760 | 2.500 |
| Rmerge | 0.052 | 0.028 | 0.446 |
| Rmeas | 0.061 | 0.033 | 0.529 |
| Rpim | 0.032 | 0.017 | 0.279 |
| Total number of observations | 66793 | ||
| Number of reflections | 20058 | ||
| <I/σ(I)> | 6 | ||
| Completeness [%] | 98.2 | 94.8 | 99.4 |
| Redundancy | 3.3 | 3.2 | 3.3 |
| CC(1/2) | 0.998 | 0.899 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 289 | 0.2M Lithium sulfate monohydrate; 0.1M BIS-TRIS 25% w/v Polyethylene glycol 3,350 |
