5CM8
Structural Basis for the Selectivity of Guanine Nucleotide Exchange Factors for the small G-protein Ral
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-11-25 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.95372 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 111.110, 98.490, 71.320 |
| Unit cell angles | 90.00, 91.61, 90.00 |
Refinement procedure
| Resolution | 44.420 - 2.600 |
| R-factor | 0.26209 |
| Rwork | 0.259 |
| R-free | 0.32188 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4JGW as poly Ala model |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.927 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.700 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.096 | |
| Number of reflections | 23162 | |
| <I/σ(I)> | 15.81 | 3.21 |
| Completeness [%] | 97.7 | 99.6 |
| Redundancy | 3.1 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.45 | 289 | 15% PEG 3350, 200 mM ammonium acetate, 100 mM Bis-Tris propane |
