5CKQ
CUB1-EGF-CUB2 domains of rat MASP-1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-05-13 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | I 2 3 |
| Unit cell lengths | 152.710, 152.710, 152.710 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 76.355 - 3.704 |
| R-factor | 0.25 |
| Rwork | 0.247 |
| R-free | 0.29370 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3dem |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.751 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 76.400 | 4.140 |
| High resolution limit [Å] | 3.700 | 3.700 |
| Rmerge | 0.742 | |
| Number of reflections | 6473 | |
| <I/σ(I)> | 9.2 | 2.1 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 5.6 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 100 mM imidazole/MOPS pH 6.5 containing 20% ethylene glycol and 10% PEG8K |
