5CJS
Crystal structure of a monomeric influenza hemagglutinin stem in complex with an broadly neutralizing antibody CR9114
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-04-29 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97950 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 110.869, 110.869, 359.161 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 48.010 - 4.300 |
R-factor | 0.264 |
Rwork | 0.262 |
R-free | 0.30300 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.865 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 4.370 |
High resolution limit [Å] | 4.300 | 4.300 |
Number of reflections | 18316 | |
<I/σ(I)> | 9.1 | 1.2 |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 8.1 | 6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 295 | 0.1 M Tris, pH 8.0, 0.2 M disodium hydrogen phosphate, 22% w/v PEG4000 |