5CJE
Structure of CYP107L2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 5C (4A) |
| Synchrotron site | PAL/PLS |
| Beamline | 5C (4A) |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-07-22 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.97952 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 110.184, 50.585, 74.638 |
| Unit cell angles | 90.00, 98.91, 90.00 |
Refinement procedure
| Resolution | 36.870 - 2.500 |
| R-factor | 0.17435 |
| Rwork | 0.170 |
| R-free | 0.25545 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2cd8 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.624 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.540 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.474 | |
| Number of reflections | 14255 | |
| <I/σ(I)> | 30.5 | 7.8 |
| Completeness [%] | 99.2 | 100 |
| Redundancy | 5.3 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 287 | Sodium Citrate, Imidazole |
