5CJ8

Human Osteoclast Associated Receptor (OSCAR) extracellular domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2013-01-06
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0000
Spacegroup name	P 1 21 1
Unit cell lengths	27.215, 46.464, 66.980
Unit cell angles	90.00, 93.25, 90.00

Refinement procedure

Resolution	33.436 - 2.015
R-factor	0.2247
Rwork	0.223
R-free	0.25540
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1g0x 1b6u
RMSD bond length	0.009
RMSD bond angle	1.328
Refinement software	PHENIX (1.7.3_928)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.000
Number of reflections	10875
<I/σ(I)>	20.3
Completeness [%]	99.8
Redundancy	5.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291.15	0.2 M potassium fluoride, 20% w/v polyethylene glycol 3,350