5CJ2
Ran GDP Y39A mutant triclinic crystal form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-02-19 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.999989 |
| Spacegroup name | P 1 |
| Unit cell lengths | 59.190, 80.730, 91.650 |
| Unit cell angles | 97.66, 90.00, 90.01 |
Refinement procedure
| Resolution | 47.579 - 1.750 |
| R-factor | 0.19877 |
| Rwork | 0.197 |
| R-free | 0.23913 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | human Ran GDP |
| RMSD bond length | 0.019 |
| RMSD bond angle | 2.117 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.579 | 1.800 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.037 | 0.368 |
| Number of reflections | 156462 | |
| <I/σ(I)> | 14.42 | 2.46 |
| Completeness [%] | 92.2 | 81.5 |
| Redundancy | 2.02 | 1.73 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 291 | 35% PEG 1000, 50mM Tris pH 8.5, 20mM MgCl2, 1 mM BeF |
