5CIF
Complex of yeast cytochrome c peroxidase (W191F) with iso-1 cytochrome c
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-10-04 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9778 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.797, 113.842, 88.239 |
| Unit cell angles | 90.00, 105.31, 90.00 |
Refinement procedure
| Resolution | 43.432 - 2.010 |
| R-factor | 0.2177 |
| Rwork | 0.216 |
| R-free | 0.26750 |
| Data scaling software | HKL-2000 |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.030 |
| High resolution limit [Å] | 2.000 | 5.430 | 2.000 |
| Rmerge | 0.222 | 0.251 | 0.488 |
| Total number of observations | 173068 | ||
| Number of reflections | 51828 | ||
| <I/σ(I)> | 14.9 | ||
| Completeness [%] | 92.2 | 91.9 | 90.7 |
| Redundancy | 3.3 | 3.3 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | PEG 3350 15-15%, 100 mM NaAcetate, 175 mM NaCl |
