5CHK
Crystal structure of avidin - HABA complex (hexagonal crystal form)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-05-30 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.977609 |
Spacegroup name | P 64 2 2 |
Unit cell lengths | 61.310, 61.310, 120.530 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 40.180 - 2.200 |
R-factor | 0.16401 |
Rwork | 0.161 |
R-free | 0.22746 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1vyo |
RMSD bond length | 0.020 |
RMSD bond angle | 2.003 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.200 |
Number of reflections | 7329 |
<I/σ(I)> | 31.92 |
Completeness [%] | 100.0 |
Redundancy | 5.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 292.15 | 0.1M sodium citrate tribasic dehydrate pH 4.5, 24% w/v polyethylene glycol 1000 |