5CHG
Human DNA polymerase lambda L431A mutant- MgdGTP binary and complex with 6 paired DNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL15A1 |
Synchrotron site | NSRRC |
Beamline | BL15A1 |
Temperature [K] | 95 |
Detector technology | CCD |
Collection date | 2013-06-29 |
Detector | RAYONIX MX300HE |
Wavelength(s) | 1.0000 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 83.893, 83.893, 398.646 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 26.892 - 2.900 |
R-factor | 0.2093 |
Rwork | 0.205 |
R-free | 0.24740 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1xsl |
RMSD bond length | 0.005 |
RMSD bond angle | 0.860 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 30.000 | 30.000 | 3.000 |
High resolution limit [Å] | 2.900 | 6.230 | 2.900 |
Rmerge | 0.170 | 0.046 | 0.879 |
Total number of observations | 272760 | ||
Number of reflections | 19664 | ||
<I/σ(I)> | 7 | ||
Completeness [%] | 100.0 | 99.6 | 100 |
Redundancy | 13.9 | 12.2 | 14.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 0.1M Lithium Sulfate monohydrate, 0.1M HEPES-Sodium, 0.1M Potassium Sodium Tartrate |