5CGP
Selective pharmacological inhibition of the CREB binding protein bromodomain regulates inflammatory cytokines in macrophages and RGS4 in neurons
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-09-05 |
Detector | RIGAKU SATURN 944 |
Wavelength(s) | 1.54178 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 90.090, 33.740, 40.010 |
Unit cell angles | 90.00, 92.70, 90.00 |
Refinement procedure
Resolution | 31.590 - 1.960 |
R-factor | 0.213 |
Rwork | 0.209 |
R-free | 0.24930 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3p1f |
RMSD bond length | 0.010 |
RMSD bond angle | 1.070 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | BUSTER |
Refinement software | BUSTER (2.11.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 31.595 | 1.912 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.141 | 0.675 |
Number of reflections | 9495 | |
<I/σ(I)> | 10.8 | 1.2 |
Completeness [%] | 98.2 | 76.5 |
Redundancy | 5.6 | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 15-20% PEG 8000, 0.2M CaAcetate, 0.1 M cacodylate ph 6-7 |