5CG8
NgTET1 in complex with 5hmC DNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-BM |
Synchrotron site | APS |
Beamline | 22-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-03-09 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.0000 |
Spacegroup name | I 21 21 21 |
Unit cell lengths | 83.802, 107.360, 167.671 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 27.554 - 2.702 |
R-factor | 0.1928 |
Rwork | 0.191 |
R-free | 0.22950 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4lt5 |
RMSD bond length | 0.006 |
RMSD bond angle | 0.892 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 27.600 | 2.790 |
High resolution limit [Å] | 2.690 | 2.690 |
Rmerge | 0.066 | 0.977 |
Number of reflections | 20985 | |
<I/σ(I)> | 29.2 | 2.9 |
Completeness [%] | 99.7 | 100 |
Redundancy | 9.9 | 10 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 289 | 25% (w/v) polyethylene glycol monomethyl ether 550, 10 mM ZnSO4, and 100 mM MES, pH 6.5 |