5CFQ
Crystal structure of anemone STING (Nematostella vectensis) in complex with 2',3' cGAMP, c[G(2',5')pA(3',5')p]
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 80 |
Detector technology | CCD |
Collection date | 2015-02-12 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.11587 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 40.497, 77.971, 101.347 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.985 - 2.099 |
R-factor | 0.2432 |
Rwork | 0.242 |
R-free | 0.25880 |
Structure solution method | SAD |
RMSD bond length | 0.003 |
RMSD bond angle | 0.733 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 42.490 | 2.160 |
High resolution limit [Å] | 2.099 | 2.099 |
Rmerge | 0.024 | 0.541 |
Number of reflections | 19434 | |
<I/σ(I)> | 16.2 | 1.6 |
Completeness [%] | 99.6 | 95.6 |
Redundancy | 14.6 | 6.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | 500 mM LiSO4, 2% PEG-8000 |