5CFH
human beta-2 microglobulin double mutant W60G-Y63W
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 110 |
Detector technology | PIXEL |
Collection date | 2014-03-02 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.99 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 93.346, 29.090, 44.410 |
Unit cell angles | 90.00, 113.01, 90.00 |
Refinement procedure
Resolution | 25.110 - 1.490 |
R-factor | 0.17198 |
Rwork | 0.169 |
R-free | 0.22961 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2z9t |
RMSD bond length | 0.016 |
RMSD bond angle | 1.752 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.110 | 1.570 |
High resolution limit [Å] | 1.490 | 1.490 |
Rmerge | 0.075 | 0.220 |
Number of reflections | 17435 | |
<I/σ(I)> | 9.6 | 4.5 |
Completeness [%] | 95.8 | 96.5 |
Redundancy | 3.3 | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | PEG 10000, HEPES |