5CF9
Cleavage of nicotinamide adenine dinucleotide by the ribosome inactivating protein of Momordica charantia - enzyme-NADP+ co-crystallisation.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-05-17 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.917 |
Spacegroup name | H 3 |
Unit cell lengths | 130.510, 130.510, 37.980 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 37.675 - 1.520 |
R-factor | 0.1398 |
Rwork | 0.138 |
R-free | 0.16800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1mom |
RMSD bond length | 0.008 |
RMSD bond angle | 1.100 |
Data reduction software | XDS |
Data scaling software | xia2 |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 62.500 | 1.560 |
High resolution limit [Å] | 1.520 | 1.520 |
Rmerge | 0.058 | 0.910 |
Number of reflections | 37103 | |
<I/σ(I)> | 15.3 | 1.5 |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 7.9 | 5.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 293 | PEG 4000, sodium phosphate |