5CEN

Crystal structure of DLK (kinase domain)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	100
Detector technology	CCD
Collection date	2010-05-05
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97740
Spacegroup name	P 1 21 1
Unit cell lengths	57.289, 39.713, 59.907
Unit cell angles	90.00, 104.73, 90.00

Refinement procedure

Resolution	46.360 - 1.700
R-factor	0.1871
Rwork	0.185
R-free	0.22460
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3dtc
RMSD bond length	0.010
RMSD bond angle	1.020
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	BUSTER-TNT (2.9.1)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	75.000	75.000	1.760
High resolution limit [Å]	1.700	3.660	1.700
Rmerge	0.072	0.040	0.629
Total number of observations	106694
Number of reflections	29149
<I/σ(I)>	9.2
Completeness [%]	100.0	99.9	100
Redundancy	3.7	3.7	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.7	292	0.2 M magnesium acetate, 20% PEG 3350, 0.1 M HEPES pH 7.7