5CDX

Crystal structure of conserpin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2010-12-16
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.953690
Spacegroup name	C 2 2 21
Unit cell lengths	68.140, 76.120, 150.240
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.060 - 2.400
R-factor	0.1975
Rwork	0.194
R-free	0.25820
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ne4
RMSD bond length	0.007
RMSD bond angle	1.025
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.7.3_928)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.060	2.486
High resolution limit [Å]	2.400	2.400
Rmerge	0.045
Number of reflections	15325
<I/σ(I)>	9.2	2.16
Completeness [%]	97.8	97.46
Redundancy	2	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	288	Crystals appeared within 5 days in 0.2 M magnesium chloride hexahydrate, 16% PEG-3350, 10 mM bis-tris, pH 7.5