5CD1

Structure of an asymmetric tetramer of human tRNA m1A58 methyltransferase in a complex with SAH and tRNA3Lys

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	103.15
Detector technology	CCD
Collection date	2014-09-05
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.11587
Spacegroup name	P 21 21 21
Unit cell lengths	128.240, 137.440, 157.590
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	59.392 - 3.600
R-factor	0.2337
Rwork	0.232
R-free	0.27290
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5ccb
RMSD bond length	0.004
RMSD bond angle	0.763
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.8.1_1168)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	102.300	3.690
High resolution limit [Å]	3.600	3.600
Rmerge	0.308	1.870
Number of reflections	32882
<I/σ(I)>	6.4	1
Completeness [%]	99.3	99.8
Redundancy	4.3	4.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	289	1 microliter protein solution (4.8 mg/ml protein, 66.4 micromolar tRNA3Lys, 2mM SAH, 1mM MgCl2 and 50mM Hepes pH7.5) mixed with 1 microliter reservoir solution (0.1M NaAcetate, pH4.8-5.0,2% w/v PEG 4000, and 15% v/v MPD) over 1000 microliter reservoir solution