5CCU
Crystal structure of endoglycoceramidase I from Rhodococ-cus equi
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-06-26 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.979 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 192.841, 48.980, 120.304 |
Unit cell angles | 90.00, 114.30, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.110 |
R-factor | 0.186 |
Rwork | 0.184 |
R-free | 0.21800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2osw |
RMSD bond length | 0.005 |
RMSD bond angle | 1.073 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.180 |
High resolution limit [Å] | 2.108 | 2.100 |
Number of reflections | 58536 | |
<I/σ(I)> | 10.09 | |
Completeness [%] | 98.4 | 96 |
Redundancy | 4.2 | 4.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 294 | PEG 6000 MES sodium hydroxide Ammonium chloride |