5CBZ
AncMR DNA Binding Domain - (+)GRE Complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-07-20 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.532, 81.150, 116.416 |
| Unit cell angles | 90.00, 96.80, 90.00 |
Refinement procedure
| Resolution | 25.003 - 2.200 |
| R-factor | 0.1974 |
| Rwork | 0.196 |
| R-free | 0.23000 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.225 |
| Data reduction software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 40.000 |
| High resolution limit [Å] | 2.200 |
| Number of reflections | 44748 |
| <I/σ(I)> | 14.7 |
| Completeness [%] | 98.8 |
| Redundancy | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 0.1 M MES, 20% PEG 6000, and 5% glycerol |
