5CBX
AncGR DNA Binding Domain - (+)GRE Complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-10-22 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.00 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 47.820, 81.575, 116.520 |
Unit cell angles | 90.00, 97.19, 90.00 |
Refinement procedure
Resolution | 33.326 - 2.000 |
R-factor | 0.2114 |
Rwork | 0.210 |
R-free | 0.22910 |
RMSD bond length | 0.006 |
RMSD bond angle | 0.831 |
Data reduction software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 40.000 |
High resolution limit [Å] | 2.000 |
Number of reflections | 57251 |
<I/σ(I)> | 14.5 |
Completeness [%] | 95.4 |
Redundancy | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.1 M HEPES (pH 7.5), 12% PEG 20000, and 5% glycerol |