5CBX
AncGR DNA Binding Domain - (+)GRE Complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-10-22 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.820, 81.575, 116.520 |
| Unit cell angles | 90.00, 97.19, 90.00 |
Refinement procedure
| Resolution | 33.326 - 2.000 |
| R-factor | 0.2114 |
| Rwork | 0.210 |
| R-free | 0.22910 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.831 |
| Data reduction software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 40.000 |
| High resolution limit [Å] | 2.000 |
| Number of reflections | 57251 |
| <I/σ(I)> | 14.5 |
| Completeness [%] | 95.4 |
| Redundancy | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.1 M HEPES (pH 7.5), 12% PEG 20000, and 5% glycerol |
