5CBE
E10 in complex with CXCL13
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 193 |
| Detector technology | PIXEL |
| Collection date | 2013-04-10 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 125.000, 125.000, 98.000 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.920 - 2.400 |
| R-factor | 0.2495 |
| Rwork | 0.248 |
| R-free | 0.27460 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | E10 and 3GV3/2R3Z/4HSV/3IL8 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.060 |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.070 | 0.605 |
| Number of reflections | 34874 | |
| <I/σ(I)> | 30.4 | 3.6 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 11.6 | 9.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 291 | 100 mM KH2PO4, 100 mM NaH2PO4, 100 mM MES pH 6, 2000 mM NaCl |
