5CB5
Structural Insights into the Mechanism of Escherichia coli Ymdb
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-03-26 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 289.140, 289.140, 114.053 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 38.720 - 2.800 |
| R-factor | 0.26296 |
| Rwork | 0.262 |
| R-free | 0.29036 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1spv |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.896 |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.850 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.114 | 0.386 |
| Number of reflections | 133057 | |
| <I/σ(I)> | 12.7 | 3.1 |
| Completeness [%] | 98.9 | 98.9 |
| Redundancy | 3.6 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 285 | 2 M ammonium sulfate, 0.1 M BIS-TRIS |
